关键词: 肺功能, 大鼠, 环境, 体质量

Abstract: Objective To develop a stable and reliable method for pulmonary function testing in rats. Method Before the experiments, the small animal pulmonary function test system was calibrated. Lung function indices of rats were measured by the system after tracheal intubation. The influence of environmental factors and body weight on lung function indices was evaluated. Result Environmental factors, including changes of air pressure or airflow, can influence the pressure sensor and alter the waveform. The rats with heavier body weights (340-370) g showed significantly increased functional residual capacity ( FRC ) , dynamic compliance ( Cdyn ) , inspiratory resistance (RI) , forced expiratory volume in 100 ms ( FEV100) , forced vital capacity ( FVC) and mean mid expiratory flow (MMEF) when compared to the rats with lower body weights ( 280 - 300) g. However, the body weights of rats did not show significant effects on inspiratory resistance ( RI) . Conclusion An easy and reliable method was successfully established for pulmonary function testing in rats. This experiment should be performed in a stable environment, and the body weights between different groups should be consistent. 

Key words: pulmonary function, rats, environment, body weight